| Rank | Top 10 Largest Zoos in the United States |
| 1 | The Columbus Zoo and Aquarium Area: 580 acres Year Opened: 1927 Location: Ohio |
| 2 | Disney’s Animal Kingdom Area: 580 acres Year Opened: 1998 Location: Florida |
| 3 | Minnesota Zoo Area: 485 acres Year Opened: 1978 Location: Minnesota |
| 4 | Bronx Zoo Area: 265 acres Year Opened: 1899 Location: New York |
| 5 | Brookfield Zoo Area: 215 acres Year Opened: 1934 Location: Chicago, IL |
| 6 | Riverbanks Zoo & Garden Area: 170 acres Year Opened: 1974 Location: South Carolina |
| 7 | Smithsonian National Zoological Park Area: 163 acres Year Opened: 1889 Location: Washington D.C. |
| 8 | Los Angeles Zoo Area: 133 acres Year Opened: 1966 Location: California |
| 9 | Henry Doorly Zoo Area: 130 acres Year Opened: 1894 Location: Nebraska |
| 10 | Detroit Zoo Area: 125 acres Year Opened: 1928 Location: Michigan |
